Mouse Matrix Metalloproteinase 13 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Matrix Metalloproteinase 13 in biofluid samples, such as serum, plasma and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . MMP13 present in samples reacts with an anti-Mouse MMP13 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-MMP13 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active MMP13 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse MMP13. Detection Target: Mouse Matrix Metalloproteinase 13 Reactivity: Mouse Range: 78.1-5000pg/mL Sensitivity: 36pg/mL Storage Conditions: Store all components at 4┬░C This Mouse MMP13 activity assay is for the quantitative determination of Matrix Metalloproteinase 13 in samples including serum, plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.